Seasonal variation in total phenolic and flavonoid contents and DPPH scavenging activity of Bellis perennis L. flowers.
نویسندگان
چکیده
Variations in total phenolic and flavonoid contents as well as antioxidant activity of Bellis perennis (common daisy) flowers were investigated. The flowers were collected monthly (from March to October, i.e., during the usual flowering season of the plant) at three localities in three different years. Total flavonoids were determined spectrophotometrically by two methods: by formation of a complex with aluminium chloride after acidic hydrolysis of flower extracts (method 1) and by reaction with boric and oxalic acids in extracts without their modification (method 2). Total phenolics were determined spectrophotometrically using the Folin-Ciocalteu reagent. The antioxidant activity was determined spectrophotometrically by a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The contents of flavonoids varied from 0.31 to 0.44 mg quercetin equivalent/100 mg dry weight (method 1) and from 1.37 to 2.20 mg pigenin-7-glucoside equivalent/100 mg dry weight (method 2). Total phenolics ranged from 2.81 to 3.57 mg gallic acid equivalent/100 mg dry weight. The antioxidant activity expressed as IC(50) values varied from 66.03 to 89.27 µg/mL; it is about 50, 30, 20, and 10 times lower as compared with quercetin, ascorbic acid, Trolox®, and butylhydroxytoluene, respectively, and about five times higher in comparison with apigenin-7-glucoside. There is a significant correlation between antioxidant activity and total phenolics. No correlation between total flavonoid contents and antioxidant activity was observed. Contents of phenolics and flavonoids as well as antioxidant activity of daisy flowers vary to a relatively small extent during the year and are not dependant on the time of collection. Thus, the flowers possess comparable quality as to these characteristics over the whole flowering season of Bellis perennis. Effects of environmental factors on the amounts of secondary metabolites in plants are also discussed.
منابع مشابه
Total Phenolic and Flavonoids Contents, Radical Scavenging Activity and Green Synthesis of Silver Nanoparticles by Laurus nobilis L. Leaves Aqueous Extract
In this project, total phenolic and flavonoid contents of the aqueous extract of Laurus nobilis L. leaves were evaluated by colorimetric methods. These natural products which are found in plants extracts, can be considered as reducing and stabilizing agents in the synthesis of metal nanoparticles. Regarding to high amounts of these compounds in the extract, silver nanoparticles were synthesized...
متن کاملAntioxidant Activity, Total Phenolics and Flavonoid Contents of Some Edible Green Seaweeds from Northern Coasts of the Persian Gulf
The antioxidant activity, contents of total phenolics and flavonoids were quantified in the methanolic extracts of four Ulva species(U.clathrata,U.linza,U.flexuosa and U.intestinalis) grown at different parts of northern coasts of the Persian Gulf in south of Iran.The seaweeds were collected from Dayyer,Taheri and Northern Ouli coasts in April 2011. Methanolic extracts of the seaweeds were asse...
متن کاملAntioxidant Activity, Total Phenolics and Flavonoid Contents of Some Edible Green Seaweeds from Northern Coasts of the Persian Gulf
The antioxidant activity, contents of total phenolics and flavonoids were quantified in the methanolic extracts of four Ulva species(U.clathrata,U.linza,U.flexuosa and U.intestinalis) grown at different parts of northern coasts of the Persian Gulf in south of Iran.The seaweeds were collected from Dayyer,Taheri and Northern Ouli coasts in April 2011. Methanolic extracts of the seaweeds were asse...
متن کاملAntioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan. Somaye Fattahi, Rashid Jamei* and Siavash Hosseini Sarghein
Rosa canina L. is a member of Rosaceae family, which is well-known for its high phenolic contents. These compounds are known to possess antioxidant and antiradical effects. In order to compare the antioxidant and antiradical activity of wild Rosa phenolic extracts, two wild Rosa species (Rosa canina L. and Rosa pimpinellifolia L.) were selected from Takab, Oshnavieh and Qasemloo Valley of Urmia...
متن کاملAntioxidant Activity, Phenolic and Flavonoid Contents of Echium Species from Different Geographical Locations of Iran
Iranian Echium species (Boraginaceae), popularly known as "Gol-e-Gavzaban" are native plants that some of them have been used widely as food and traditional medicine since long times. In this work organs from different populations of two Iranian Echium species were collected from their natural locations in order to analyze their phenolic content and antioxidant activity. Hydroalcoholic extracts...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecules
دوره 15 12 شماره
صفحات -
تاریخ انتشار 2010